期刊
MICROBIOLOGYOPEN
卷 8, 期 1, 页码 -出版社
WILEY
DOI: 10.1002/mbo3.624
关键词
chlamydospore; Clonostachys rosea; differentially expressed gene; reverse transcription quantitative PCR; transcriptome
类别
资金
- National Natural Science Foundation of China [31471815]
- Program of Modern Agricultural Industry Technology System [CARS-25-B-02]
- Special Project of Ministry of Agriculture [201503112-2]
Chlamydospores are specific structures that are of great significance to the commercialization of fungal biopesticides. To explore the genes associated with chlamydospore formation, a biocontrol fungus Clonostachys rosea 67-1 that is capable of producing resistant spores under particular conditions was investigated by transcriptome sequencing and analysis. A total of 549,661,174 clean reads were obtained, and a series of differentially expressed genes potentially involved in fungal chlamydospore formation were identified. At 36 hr, 67 and 117 genes were up- and downregulated in C. rosea during chlamydospore production, compared with the control for conidiation, and 53 and 24 genes were up- and downregulated at 72 hr. GO classification suggested that the differentially expressed genes were related to cellular component, biological process, and molecular function categories. A total of 188 metabolism pathways were linked to chlamydospore production by KEGG analysis. Sixteen differentially expressed genes were verified by reverse transcription quantitative PCR, and the expression profiles were consistent with the transcriptome data. To the best of our knowledge, it is the first report on the genes associated with chlamydospore formation in C. rosea. The results provide insight into the molecular mechanisms underlying C. rosea sporulation, which will assist the development of fungal biocontrol agents.
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