期刊
MATERIALS HORIZONS
卷 5, 期 1, 页码 65-69出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7mh00804j
关键词
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资金
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- National Natural Science Foundation of China [21522505]
While DNA/graphene oxide (GO) conjugates have been widely used for DNA detection, they suffer from non-specific DNA displacement by proteins, making their application in biological samples difficult. To find new materials tightly adsorbing DNA but not proteins, we screened seven metal oxide nanoparticles, all interacting with the phosphate backbone of DNA, while DNA uses its nucleobases to interact with GO. In this regard, DNA is a Janus polymer orthogonally adsorbing GO and metal oxides. The DNA adsorption affinity ranks CoO > NiO > Cr2O3 > Fe2O3 > Fe3O4 > TiO2 > CeO2 based on a phosphate displacement assay. Among them, CoO is nearly fully resistant to protein displacement, while NiO has the best limit of detection of 0.24 nM DNA. This study provides fundamental insights into the biointerface chemistry of DNA, and reveals new materials useful for bioanalytical chemistry, DNA separation, and DNA-directed assembly.
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