期刊
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
卷 -, 期 136, 页码 -出版社
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/57949
关键词
Biochemistry; Issue 136; Leaf spray MS; mass spectrometry; electrospray ionization; ambient ionization; Sceletium tortuosum; mesembrine alkaloids; natural products; plant metabolites; small molecules
资金
- NSF Plant Genome Research Program [IOS-1238812]
- NSF Postdoctoral Fellowship in Biology [IOS-1400818]
- Monsanto Graduate Student Fellowship
- Fulbright African Researcher Scholars Program
Plants produce thousands of small molecules that are diverse in their chemical properties. Mass spectrometry (MS) is a powerful technique for analyzing plant metabolites because it provides molecular weights with high sensitivity and specificity. Leaf spray MS is an ambient ionization technique where plant tissue is used for direct chemical analysis via electrospray, eliminating chromatography from the process. This approach to sampling metabolites allows for a wide range of chemical classes to be detected simultaneously from intact plant tissues, minimizing the amount of sample preparation needed. When used with a high-resolution, accurate mass MS, leaf spray MS facilitates the rapid detection of metabolites of interest. It is also possible to collect tandem mass fragmentation data with this technique to facilitate a compound identification. The combination of accurate mass measurements and fragmentation is beneficial in confirming compound identities. The leaf spray MS technique requires only minor modifications to a nanospray ionization source and is a useful tool to further expand the capabilities of a mass spectrometer. Here, fresh leaf tissue from Sceletium tortuosum (Aizoaceae), a traditional medicinal plant from South Africa, is analyzed; numerous mesembrine alkaloids are detected with leaf spray MS.
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