期刊
JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION
卷 41, 期 9, 页码 1097-1102出版社
SPRINGER
DOI: 10.1007/s40618-018-0836-x
关键词
Liraglutide; Skeletal muscle insulin resistance; IRS1 serine phosphorylation
资金
- National Natural Science Foundation of China [81670731]
- Tianjin Natural Science Foundation [14JCY-BJC26200]
- Tianjin Municipal Science and Technology Commission [15JCZDJC35500]
- Tianjin Health and Family Planning Commission [15KG102, 15ZXHLSY00460]
Objective A reduction in insulin-stimulated glucose uptake in skeletal muscles is a characteristic of insulin resistance and type 2 diabetes mellitus (T2DM). The glucagon-like peptide (GLP)-1 agonist liraglutide can reduce blood glucose levels in individuals with T2DM. However, its effect on insulin-induced glucose metabolism in the skeletal muscle of insulin resistance is unknown. We investigated the effects and action mechanisms of liraglutide on insulin resistance (IR) in the skeletal muscle cells treatment with palmitic acid (PA). Methods The cell-surface GLUT4myc levels were determined by an antibody-coupled colorimetric assay. The phosphorylation levels of Akt, PI3K(p85 alpha), AS160, IRS1, IKK, and JNK were determined by western blotting. The quantifications of mRNA levels of TNF alpha, IL-1 beta, and IL-6 were determined by real-time PCR. Analysis of variance was used for data analysis. Results PA elevated not only phosphorylation of JNK, IRS1 serines, and IKK alpha/beta, but also the expression of IL-6, TNF alpha and IL-1 beta in C2C12-GLUT4myc cells. PA can reduce phosphorylation of IRS1 tyrosine. These effects of PA were reversed by liraglutide. In addition, liraglutide can reverse PA-decreased insulin-stimulated cell-surface GLUT4 levels, Akt, PI3K(p85 alpha), and AS160 phosphorylation. Conclusions Liraglutide can enhance insulin-induced GLUT4 translocation by inhibiting IRS1 serine phosphorylation in PA-treated muscle cells.
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