期刊
JOURNAL OF CO2 UTILIZATION
卷 25, 期 -, 页码 264-274出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.jcou.2018.03.017
关键词
Porcine acellular dermal matrix; Epoxy compound; Cross-linking; Supercritical CO2 fluid; Wound healing
资金
- National Natural Science Foundation of China [51473001]
- key technology research and development program of Jiangyin city [JYKJ3369]
In addition to the cross-linker, which has an impact on the properties of the materials being cross-linked, the reaction media can impact the reaction's effectiveness. In this study, porcine acellular dermal matrix (pADM), an aggregation of collagen fibers, was cross-linked by ethylene glycol diglycidyl ether (EGDE) in supercritical CO2 fluid (sc-CO2) reaction medium (EGDE-pADM). And pADM cross-linked in ethyl alcohol medium (EtOH-EGDE-pADM) was used as control. The physicochemical properties and biocompatibility of the cross-linked pADM were evaluated. The results showed that EGDE-pADM had improved thermal stability, hydrophilicity, mechanical strength, resistance to enzymatic degradation and pore structure compared with the uncross-linked pADM and EtOH- EGDE-pADM. And the improvement of scaffolds' properties was related to the pressure of sc-CO2. The EGDE-pADM had excellent biocompatibility both in vitro and in vivo; it improved the adhesion and proliferation of L929 fibroblast cells, especially when compared with EtOH-EGDE-pADM using organic solvent, and expedited the wound healing process. These findings indicate that the reaction medium had a certain influence on the properties and cytotoxicity of the cross-linked pADM, sc-CO2 fluid was proven to be a safe and reliable cross-linking reaction medium for collagen.
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