4.6 Article

Somatostatin Analogue Treatment Primarily Induce miRNA Expression Changes and Up-Regulates Growth Inhibitory miR-7 and miR-148a in Neuroendocrine Cells

期刊

GENES
卷 9, 期 7, 页码 -

出版社

MDPI
DOI: 10.3390/genes9070337

关键词

somatostatin analogues; neuroendocrine tumors; cancer; miRNAs; miR-148a; miR-7; let-7

资金

  1. Lundbeck Foundation [R31-A2394]
  2. Desiree and Niels Yde Foundation
  3. Danish National Research Foundation
  4. Novo Nordisk Foundation
  5. National Advanced Technology Foundation [005-2007-2]
  6. Svend Andersen Foundation (2008)
  7. Rigshospitalets Research Council [604-18]
  8. Capital Region of Denmark [R101-A1945]

向作者/读者索取更多资源

Somatostatin (SST) analogues are used to control the proliferation and symptoms of neuroendocrine tumors (NETs). MicroRNAs (miRNA) are small non-coding RNAs that modulate posttranscriptional gene expression. We wanted to characterize the miRNAs operating under the control of SST to elucidate to what extent they mediate STT actions. NCI-H727 carcinoid cell line was treated with either a chimeric SST/dopamine analogue; a SST or dopamine analogue for proliferation assays and for identifying differentially expressed miRNAs using miRNA microarray. The miRNAs induced by SST analogue treatment are investigated in carcinoid cell lines NCI-H727 and CNDT2 using in situ hybridization, qPCR and proliferation assays. SST analogues inhibited the growth of carcinoid cells more potently compared to the dopamine analogue. Principal Component Analysis (PCA) of the samples based on miRNA expression clearly separated the samples based on treatment. Two miRNAs which were highly induced by SST analogues, miR-7 and miR-148a, were shown to inhibit the proliferation of NCI-H727 and CNDT2 cells. SST analogues also produced a general up-regulation of the let-7 family members. SST analogues control and induce distinct miRNA expression patterns among which miR-7 and miR-148a both have growth inhibitory properties.

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