期刊
FRONTIERS IN PHYSIOLOGY
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fphys.2018.00751
关键词
Macrobrachium nipponense; AMPK; hypoxia; energy metabolism; aquaculture
类别
资金
- National Natural Science Foundation of China [31672633]
- China Central Governmental Research Institutional Basic Special Research Project from Public Welfare Fund [2017JBFM02]
- Open Funding Project of the Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources [K2016-02]
- Science & Technology Supporting Program of Jiangsu Province [BE2012334]
- Three New Projects of Jiangsu Province [D2013-6]
Hypoxia has important effects on biological activity in crustaceans, and modulation of energy metabolism is a crucial aspect of crustaceans' ability to respond to hypoxia. The adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) enzyme is very important in cellular energy homeostasis; however, little information is known about the role of AMPK in the response of prawns to acute hypoxia. In the present study, three subunits of AMPK were cloned from the oriental river prawn, Macrobrachium nipponense. The full-length cDNAs of the alpha, beta, and gamma AMPK subunits were 1,837, 3,174, and 3,773 bp long, with open reading frames of 529, 289, and 961 amino acids, respectively. Primary amino acid sequence alignment of these three subunits revealed conserved similarity between the functional domains of the M. nipponense AMPK protein with AMPK proteins of other animals. The expression of the three AMPK subunits was higher in muscle tissue than in other tissues. Furthermore, the mRNA expression of AMPK alpha, AMPK beta, and AMPK gamma were significantly up-regulated in M. nipponense muscle tissue after acute hypoxia. Probing with a phospho-AMPK alpha antibody revealed that AMPK is phosphorylated following hypoxia; this phosphorylation event was found to be essential for AMPK activation. Levels of glucose and lactic acid in hemolymph and muscle tissue were significantly changed over the course of hypoxia and recovery, indicating dynamic changes in energy metabolism in response to hypoxic stress. The activation of AMPK by hypoxic stress in M. nipponense was compared to levels of muscular AMP, ADP, and ATP, as determined by HPLC; it was found that activation of AMPK may not completely correlate with AMP: ATP ratios in prawns under hypoxic conditions. These findings confirm that the alpha, beta, and gamma subunits of the prawn AMPK protein are regulated at the transcriptional and protein levels during hypoxic stress to facilitate maintenance of energy homeostasis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据