期刊
ENVIRONMENTAL SCIENCE-NANO
卷 5, 期 2, 页码 279-288出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7en00832e
关键词
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资金
- University of Minnesota Biotechnology Training Grant Program through the National Institutes of Health [5 T32 CM 8347-24]
- University of Minnesota UROP award
- NSF through the MRSEC program
- National Science Foundation through XSEDE resources on Bridges [TG-CTS090079]
- National Science Foundation under the Center for Sustainable Nanotechnology [CHE-1503408]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [T32GM008347] Funding Source: NIH RePORTER
We aim to establish the effect of environmental diversity in evaluating nanotoxicity to bacteria. We assessed the toxicity of 4 nm polyallylamine hydrochloride-wrapped gold nanoparticles (PAH AuNPs) to a panel of bacteria from diverse environmental niches. The bacteria experienced a range of toxicities as evidenced by the different minimum bactericidal concentrations determined; the sensitivities of the bacteria was A. vinelandii = P. aeruginosa > S. oneidensis MR-4 > A. baylyi > S. oneidensis MR-1. Interactions between gold nanoparticles and molecular components of the cell wall were investigated by TEM, flow cytometry, and computational modeling. Binding results showed a general trend that bacteria with smooth lipopolysaccharides (LPS) bind more PAH AuNPs than bacteria with rough LPS. Computational models reveal that PAH migrates to phosphate groups in the core of the LPS structure. Overall, our results demonstrate that simple interactions between nanoparticles and the bacterial cell wall cannot fully account for observed trends in toxicity, which points to the importance of establishing more comprehensive approaches for modeling environmental nanotoxicity.
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