4.2 Article

Revealing proteins associated with symbiotic germination of Gastrodia elata by proteomic analysis

期刊

BOTANICAL STUDIES
卷 59, 期 -, 页码 -

出版社

SPRINGEROPEN
DOI: 10.1186/s40529-018-0224-z

关键词

Gastrodia elata; Mycorrhizal fungi; Symbiotic germination; Plant defense

资金

  1. CAMS basic research fund for central public research institutes [2016ZX350062]
  2. Program for Innovative Research Team in IMPLAD [PIRTI-IT 1302]

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Background: Gastrodia elata, a mycoheterotrophic orchid, is a well-known medicinal herb. In nature, the seed germination of G. elata requires proper fungal association, because of the absence of endosperm. To germinate successfully, G. elata obtains nutrition from mycorrhizal fungi such as Mycena. However, Mycena is not able to supply nutrition for the further development and enlargement of protocorms into tubers, flowering and fruit setting of G. elata. To date, current genomic studies on this topic are limited. Here we used the proteomic approach to explore changes in G. elata at different stages of symbiotic germination. Results: Using mass spectrometry, 3787 unique proteins were identified, of which 599 were classified as differentially accumulated proteins. Most of these differentially accumulated proteins were putatively involved in energy metabolism, plant defense, molecular signaling, and secondary metabolism. Among them, the defense genes (e.g., pathogenesis-/wound-related proteins, peroxidases, and serine/threonine-protein kinase) were highly expressed in late-stage protocorms, suggesting that fungal colonization triggered the significant defense responses of G. elata. Conclusions: The present study indicated the metabolic change and defensive reaction could disrupt the balance between Mycena and G. elata during mycorrhizal symbiotic germination.

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