期刊
STEM CELLS INTERNATIONAL
卷 2018, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2018/4069032
关键词
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资金
- National Natural Science Foundation of China [81671608, 81202350, 81571586]
- Jiangsu Six Talent Peaks Project [2015-WSN-074]
- Jiangsu 333 High Level Talents Project
- Jiangsu Government Scholarship for Overseas Studies
- Jiangsu Health International Exchange Program sponsorship
- Jiangsu Young Medical Talents Project
Objective. To investigate the effects of umbilical cord mesenchymal stem cell (UC-MSC) transplantation on joint damage and osteoporosis in collagen-induced arthritis (CIA) mice and to explore the mechanisms by which UC-MSCs modulate the osteogenic differentiation. Methods. CIA mice were divided into the following treated groups: UC-MSC transplantation group, antitumor necrosis factor-(TNF-) alpha group, and zoledronic acid (ZA) group. Microcomputed tomography (micro-CT) was used to analyze the bone morphology parameters. Osteogenic differentiation of treated CIA mice was determined. Bone marrow mesenchymal stem cells (BM-MSCs) from CIA mice were treated with TNF-alpha in vitro to explore their effects on osteogenesis. Results. The arthritis score was significantly reduced in the UC-MSC transplantation and anti-TNF-alpha-treated CIA groups, compared with control mice (P < 0 001). Micro-CT showed that CIA mice developed osteoporosis at 12 weeks after immunization. The bone morphology parameters were partially improved in UC-MSC-treated CIA mice. Impaired osteogenic differentiation functions were indicated by decreased ALP activity (P < 0 001) and reduced mRNA and protein levels of osteogenic marker genes (P < 0 05) in CIA mice compared with DBA/1 mice. UC-MSC treatment significantly upregulated the impaired osteogenic differentiation ability in CIA mice. Meanwhile, the serum TNF-alpha level was decreased significantly in the UC-MSC group. The osteogenesis was reduced with the addition of TNF-alpha in vitro. Conclusion. This study demonstrated that UC-MSC transplantation not only significantly improved the joint damage but also played a beneficial role in osteoporosis in CIA mice. Mechanistically, the improved osteogenic differentiation of CIA under UC-MSC treatment may be achieved by inhibition of TNF-alpha.
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