期刊
SAUDI JOURNAL OF GASTROENTEROLOGY
卷 24, 期 1, 页码 59-66出版社
MEDKNOW PUBLICATIONS & MEDIA PVT LTD
DOI: 10.4103/sjg.SJG_333_17
关键词
Bifidobacterium longum supernatant; intestinal epithelial cells; Lactobacillus acidophilus supernatant; serotonin transporter
资金
- National Natural Science Foundation of China [81570489]
Background/Aims: Probiotics play a role in relieving irritable bowel syndrome (IBS); however, the underlying mechanism is yet unclear. The aim of the study was to investigate the effects of the supernatants of Lactobacillus acidophilus and Bifidobacterium longum on the expression of serotonin transporter (SERT) messenger ribonucleic acid (mRNA) and protein. Materials and Methods: HT-29 and Caco-2 cells were treated with different concentrations of L. acidophilus and B. longum supernatants for 12 h and 24 h, respectively. SERT mRNA and proteins levels were detected by real-time polymerase chain reaction (real-time PCR) and Western-blotting. Results: The mRNA levels of SERT in HT-29 and Caco-2 cells treated with different concentrations of L. acidophilus or B. longum supernatants for 12 h and 24 h, each, were higher than that in the control groups. In addition, the expression of the protein in both cells was also upregulated, which was approximately similar to that of the corresponding mRNA. Conclusions: L. acidophilus and B. longum supernatants can upregulate SERT mRNA and protein levels in intestinal epithelial cells.
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