期刊
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
卷 25, 期 6, 页码 1220-1226出版社
ELSEVIER
DOI: 10.1016/j.sjbs.2018.02.005
关键词
Epigenetics; DNA methylation; Cell-free DNA; Retroelements; LINE-1
类别
资金
- Stiftung fur Altersforschung, Heinrich-Heine-Universitat Dusseldorf
- Diputacion Foral de Gipuzkoa, Spain [DFG10/15, DFG15/15, DFG141/16]
- Ministry of Economy and Competitiveness, Spain
- MINECO [PI16/01430, BFU 2016-7798-P]
- Ikerbasque, Basque Foundation for Science, Spain
- Deanship of Scientific Research at King Saud University [RGP-198]
Aging associated DNA hypomethylation of LINE-1 and Alu retroelements may be a crucial determinant of loss of genomic integrity, deterioration and cancer. In peripheral blood LINE-1 hypomethylation has been reported to increase during aging, but other studies did not observe significant changes. We hypothesized that these apparently inconsistent reports might relate to differences between cellular and cell-free DNA. Using the technique of idiolocal normalization of real-time methylation-specific PCR (IDLN-MSP) for genetic imbalanced DNA specimens we obtained evidence that LINE-1 hypomethylation in cell-free DNA, but not cellular DNA from peripheral blood is an epigenetic biomarker for human aging. Furthermore, hypomethylation of cell-free DNA is more extensive in smokers, suggesting that it might be used as a surrogate marker for monitoring the improvement of smoking-induced adverse effects after cancelling smoking. (C) 2018 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University.
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