期刊
LEGAL MEDICINE
卷 33, 期 -, 页码 17-22出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.legalmed.2018.04.008
关键词
Biogeographical ancestry; Japaneseplex; Japanese-specific; Single nucleotide polymorphism; SNaPshot
资金
- Japan Society for the Promotion of Science [23590849, 26460878]
- Grants-in-Aid for Scientific Research [26460878, 23590849] Funding Source: KAKEN
It is sometimes necessary to determine whether a forensic biological sample came from a Japanese person. In this study, we developed a 60-locus SNP assay designed for the differentiation of Japanese people from other East Asians using entirely and nearly Japanese-specific alleles. This multiplex assay consisted of 6 independent PCR reactions followed by single nucleotide extension. The average number and standard deviation of Japanese specific alleles possessed by an individual were 0.81 +/- 0.93 in 108 Koreans from Seoul, 8.87 +/- 2.89 in 103 Japanese from Tottori, 17.20 +/- 3.80 in 88 Japanese from Okinawa, and 0 in 220 Han Chinese from Wuxi and Changsha. The Koreans had 0-4 Japanese-specific alleles per individual, whereas the Japanese had 4-26 Japanese-specific alleles. Almost all Japanese were distinguished from the Koreans and other people by the factorial correspondence and principal component analyses. The Snipper program was also useful to estimate the degree of Japaneseness. The method described here was successfully applied to the differentiation of Japanese from non-Japanese people in forensic cases. This Japanese-specific SNP assay was named Japaneseplex.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据