期刊
JOURNAL OF CANCER
卷 9, 期 2, 页码 239-249出版社
IVYSPRING INT PUBL
DOI: 10.7150/jca.21336
关键词
Regulatory T cells; colorectal cancer; depletion; Activated T cells; CD8(+) cells
类别
资金
- CONACYT (Mexico) [280013]
- Programa de Apoyo a Proyectos de Investigacion Cientifica y Tecnologica, Direccion General de Asuntos de Personal Academico (DGAPA)-UNAM [IN220316]
- Programa de Becas Posdoctorales DGAPA-UNAM
Colorectal cancer (CRC) is the second most commonly diagnosed cancer in women and the third in men in North America and Europe. CRC is associated with inflammatory responses in which intestinal pathology is caused by different cell populations including a T cell dysregulation that concludes in an imbalance between activated T (Tact) and regulatory T (Treg) cells. Treg cells are CD4(+)Foxp3(+) cells that actively suppress pathological and physiological immune responses, contributing to the maintenance of immune homeostasis. A tumor-promoting function for Treg cells has been suggested in CRC, but the kinetics of Treg cells during CRC development are poorly known. Therefore, using a mouse model of colitis-associated colon cancer (CAC) induced by azoxymethane and dextran sodium sulfate, we observed the dynamic and differential kinetics of Treg cells in blood, spleen and mesenteric lymph nodes (MLNs) as CAC progresses, highlighting a significant reduction in Treg cells in blood and spleen during early CAC development, whereas increasing percentages of Treg cells were detected in late stages in MLNs. Interestingly, when Treg cells were decreased, Tact cells were increased and vice versa. Treg cells from late stages of CAC displayed an activated phenotype by expressing PD1, CD127 and Tim-3, suggesting an increased suppressive capacity. Suppression assays showed that T-CD4(+) and T-CD8(+) cells were suppressed more efficiently by MLN Treg cells from CAC animals. Finally, an antibody-mediated reduction in Treg cells during early CAC development resulted in a better prognostic value, because animals showed a reduction in tumor progression associated with an increased percentage of activated CD4(+)CD25(+)Foxp3-and CD8(+)CD25(+) T cells in MLNs, suggesting that Treg cells suppress T cell activation at early steps during CAC development.
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