期刊
BIOSENSORS-BASEL
卷 2, 期 1, 页码 15-31出版社
MDPI AG
DOI: 10.3390/bios2010015
关键词
electrochemical biosensor; pathogen detection; magnetic polyaniline; screen-printed carbon electrode; cyclic voltammetry
资金
- U.S. Department of Homeland Security through the National Center for Food Protection and Defense [R9106007101]
- U.S. Environmental Protection Agency [RD83300501]
Biological defense and security applications demand rapid, sensitive detection of bacterial pathogens. This work presents a novel qualitative electrochemical detection technique which is applied to two representative bacterial pathogens, Bacillus cereus (as a surrogate for B. anthracis) and Escherichia coli O157:H7, resulting in detection limits of 40 CFU/mL and 6 CFU/mL, respectively, from pure culture. Cyclic voltammetry is combined with immunomagnetic separation in a rapid method requiring approximately 1 h for presumptive positive/negative results. An immunofunctionalized magnetic/polyaniline core/shell nano-particle (c/sNP) is employed to extract target cells from the sample solution and magnetically position them on a screen-printed carbon electrode (SPCE) sensor. The presence of target cells significantly inhibits current flow between the electrically active c/sNPs and SPCE. This method has the potential to be adapted for a wide variety of target organisms and sample matrices, and to become a fully portable system for routine monitoring or emergency detection of bacterial pathogens.
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