期刊
FRONTIERS IN MICROBIOLOGY
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2018.00239
关键词
Penicillium digitatum; citral; iTRAQ; oxidative phosphorylation; reactive oxygen species
类别
资金
- National Natural Science Foundation of China [31772364, 31271964]
- Collaborative Innovation Center of New Chemical Technologies for Environmental Benignity and Efficient Resource Utilization, Research Foundation of Education Bureau of Hunan Province [15A181]
- Hunan Provincial Natural Science Foundation of China [2017JJ2247]
- Hunan Provincial Innovation Foundation for Postgraduate [CX2016B266]
Citral exhibits strong antifungal activity against Penicillium digitatum. In this study, 41 over-expressed and 84 repressed proteins in P. digitatum after 1.0 mu L/mL of citral exposure for 30 min were identified by the iTRAQ technique. The proteins were closely related with oxidative phosphorylation, the TCA cycle and RNA transport. The mitochondria' complex I, complex II, complex III, complex IV and complex V, which are involved in oxidative phosphorylation were drastically affected. Among of them, the activities of mitochondria' complex I and complex IV were apparently suppressed, whereas those of mitochondria' complex II, complex III and complex V were significantly induced. Meanwhile, citral apparently triggered a reduction in the intracellular ATP, the mitochondrial membrane potential (MMP) and glutathione content, in contrast to an increase in the glutathione S-transferase activity and the accumulation of reactive oxygen species (ROS). Addition of exogenous cysteine decreased the antifungal activity. In addition, cysteine maintained the basal ROS level, deferred the decrease of MMP and the membrane damage. These results indicate that citral inhibited the growth of p. digitatum by damaging oxidative phosphorylation and cell membranes through the massive accumulation of ROS.
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