期刊
FRONTIERS IN MICROBIOLOGY
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2018.00047
关键词
malonyl-CoA sensor; phosphorylation site mutations; acetyl-CoA carboxylase; 3-hydroxypropionic acid; Saccharomyces cerevisiae
类别
资金
- National Natural Science Foundation of China [31770101, 31470163, 31711530155]
- Key R&D Program of Shandong Province [2017GSF21110, 2015GSF121015]
Malonyl-coenzyme A (malonyl-CoA) is a critical precursor for the biosynthesis of a variety of biochemicals. It is synthesized by the catalysis of acetyl-CoA carboxylase (Acc1p), which was demonstrated to be deactivated by the phosphorylation of Snf1 protein kinase in yeast. In this study, we designed a synthetic malonyl-CoA biosensor and used it to screen phosphorylation site mutations of Acc1p in Saccharomyces cerevisiae. Thirteen phosphorylation sites were mutated, and a combination of three site mutations in Acc1p, S686A, S659A, and S1157A, was found to increase malonyl-CoA availability. ACC1(S686AS659AS1157A) expression also improved the production of 3-hydroxypropionic acid, a malonyl-CoA-derived chemical, compared to both wild type and the previously reported ACC1(S659AS1157A) mutation. This mutation will also be beneficial for other malonyl-CoA-derived products.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据