期刊
FRONTIERS IN MICROBIOLOGY
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2018.00192
关键词
antibiotic resistance; mutation; rpoB; rifampicin; Bacillus subtilis; spaceflight
类别
资金
- NASA Research Opportunities in Space Biology grants [NNX12AN70G, NNX14AT38G]
- NASA [NNX14AT38G, 674048, 69793, NNX12AN70G] Funding Source: Federal RePORTER
The effect of Bacillus subtilis exposure to the human spaceflight environment on growth, mutagenic frequency, and spectrum of mutations to rifampicin resistance (Rif(R)) was investigated. B. subtilis cells were cultivated in Biological Research in Canister-Petri Dish Fixation Units (BRIC-PDFUs) on two separate missions to the International Space Station (ISS), dubbed BRIC-18 and BRIC-21, with matching asynchronous ground controls. No statistically significant difference in either growth or in the frequency of mutation to Rif(R) was found in either experiment. However, nucleotide sequencing of the Rif(R) regions of the rpoB gene from Rif(R) mutants revealed dramatic differences in the spectrum of mutations between flight (FL) and ground control (GC) samples, including two newly discovered rpoB alleles in the FL samples (Q137R and L489S). The results strengthen the idea that exposure to the human spaceflight environment causes unique stresses on bacteria, leading to alterations in their mutagenic potential.
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