期刊
FRONTIERS IN MICROBIOLOGY
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2018.00256
关键词
antimicrobial resistance; antimicrobial resistance genes; coagulase-negative staphylococci; dairy; mastitis; non-aureus staphylococci; prevalence
类别
资金
- NSERC Industrial Research Chair in Infectious Diseases of Dairy Cattle
- Canadian Bovine Mastitis and Milk Quality Research Network (CBMQRN) program
- Dairy Farmers of Canada
- Agriculture and Agri-Food Canada through the Dairy Research Cluster 2 Program
- Alberta Livestock and Meat Agency
- Canada's Natural Sciences and Engineering Research Council (NSERC) Industrial Research Chair Program
- Alberta Innovates-Technology Futures (Alberta, Canada) Graduate Student Scholarship (GSS) program
- NSERC-CREATE in Milk Quality scholarship
- Natural Sciences and Engineering Research Council of Canada (Ottawa, ON, Canada)
- Alberta Milk (Edmonton, AB, Canada)
- Dairy Farmers of New Brunswick (Sussex, New Brunswick, Canada)
- Dairy Farmers of Nova Scotia (Lower Truro, NS, Canada)
- Dairy Farmers of Ontario (Mississauga, ON, Canada)
- Dairy Farmers of Prince Edward Island (Charlottetown, PE, Canada)
- Novalait Inc. (Quebec City, QC, Canada)
- Dairy Farmers of Canada (Ottawa, ON, Canada)
- Canadian Dairy Network (Guelph, ON, Canada)
- Agriculture and Agri-Food Canada (Ottawa, ON, Canada)
- Public Health Agency of Canada (Ottawa, ON, Canada)
- Technology PEI Inc. (Charlottetown, PE, Canada)
- Universite de Montreal (Montreal, QC, Canada)
- University of Prince Edward Island (Charlottetown, PE, Canada) through the CBMQRN (Saint-Hyacinthe, QC, Canada)
- Dairy Farmers of Manitoba (Winnipeg, MB, Canada)
- British Columbia Dairy Association (Burnaby, BC Canada)
- Westgen Endowment Fund (Milner, BC, Canada)
- CanWest DHI (Guelph, ON, Canada)
Emergence and spread of antimicrobial resistance is a major concern for the dairy industry worldwide. Objectives were to determine: (1) phenotypic and genotypic prevalence of drug-specific resistance for 25 species of non-aureus staphylococci, and (2) associations between presence of resistance determinants and antimicrobial resistance. Broth micro-dilution was used to determine resistance profiles for 1,702 isolates from 89 dairy herds. Additionally, 405 isolates were sequenced to screen for resistance determinants. Antimicrobial resistance was clearly species-dependent. Resistance to quinupristin/dalfopristin was common in Staphylococcus gallinarum (prevalence of 98%), whereas S. cohnii and S. arlettae were frequently resistant to erythromycin (prevalence of 63 and 100%, respectively). Prevalence of resistance was 10% against beta-lactams and tetracyclines. In contrast, resistance to antimicrobials critically important for human medicine, namely vancomycin, fluoroquinolones, linezolid and daptomycin, was uncommon (<1%). Genes encoding multidrug-resistance efflux pumps and resistance-associated residues in deducted amino acid sequences of the folP gene were the most frequent mechanisms of resistance, regardless of species. The estimated prevalence of the mecA gene was 17% for S. epidermidis. Several genes, including blaZ, mecA, fexA, erm, mphC, msrA, and tet were associated with drug-specific resistance, whereas other elements were not. There were specific residues in gyrB for all isolates of species intrinsically resistant to novobiocin. This study provided consensus protein sequences of key elements previously associated with resistance for 25 species of non-aureus staphylococci from dairy cattle. These results will be important for evaluating effects of interventions in antimicrobial use in Canadian dairy herds.
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