期刊
BIOMATERIALS SCIENCE
卷 6, 期 5, 页码 1007-1019出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7bm00869d
关键词
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资金
- National High Technology Research and Development Program of China (863 Program) [SS2015AA020302]
- Natural Science Foundation of China [81701024, 31370963]
- Osteology Foundation
- Fundamental Research Funds for the Central Universities [17ykpy70]
- Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme
- 100 Top Talents Program of Sun Yat-sen University
- Recruitment Program of Global Young Talent, China
A barrier membrane is a major component of guided bone regeneration (GBR), which is traditionally viewed as a physical barrier. Due to its foreign body nature, the implantation of a barrier membrane would inevitably modulate immune response and subsequently affect bone dynamics, which has long been neglected. To bridge this knowledge gap, we investigated the osteoimmunomodulatory effects of barrier collagen membranes. It is found that barrier collagen membranes elicit significant effects on modulating the osteoimmune response of macrophages, by upregulating the expression of pro-inflammatory cytokines (TNF alpha, IL-1 beta, IL-6, and IL-18) and osteogenic factors (BMP2/6, WNT10b, OSM). The modulated-osteoimmune environment was beneficial for the osteogenic differentiation of BMSCs, due to the activation of BMP, canonical WNT/beta-catenin, and OSM signalling pathways. The membrane-mediated osteoimmunomodulation was further modulated to show whether osteogenesis could be enhanced via manipulating the membrane-mediated osteoimmunomodulation. The membrane-mediated osteoimmune response was successfully tuned through coating the collagen membranes with nanometersized bioactive glass Ca2ZnSi2O7 by pulsed laser deposition, which is indicated from the change in the expression profile of inflammatory cytokines and the upregulated expression of osteogenic factors. The modulated osteoimmune environment enhanced the osteogenic differentiation of BMSCs, suggesting that collagen membranes with nanometer-sized Ca2ZnSi2O7 coating can be promising for GBR applications. These results collectively imply that barrier membranes are bioactive barriers with an osteoimmunomodulatory effect and not just physical barriers. New generation barrier membranes should be designed with a favourable osteoimmunomodulatory property.
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