期刊
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
卷 46, 期 -, 页码 800-816出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/21691401.2018.1470523
关键词
Low-level laser therapy; doped nanocomposites; green synthesis; breast cancer; reduced graphene oxide; neodymium
资金
- Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran [95/112]
- University of Tabriz
In the present research, an effective drug-free approach was developed to kill MCF7 breast cancer cells using low-level laser therapy (LLLT) combined with reduced graphene oxide (rGO)-based hybrid nanocomposites (NCs). Here, fruit extract of Rosa canina was used for the first time to obtain the rGO/ZnO, Ag-ZnO/rGO and Nd-ZnO/rGO NCs by green synthesis. Physico/photochemical properties of these NCs were evaluated using FTIR, XRD, Raman, XPS, SEM/EDX, UV-Vis, DLS and AFM. The potential of the as-synthesized NCs on ROS generating and antioxidant activity were assessed by DPPH. After optimizing the proper concentration of the NCs their anti-tumoral efficacy were evaluated by DAPI staining and MTT assay tests for laser therapy on MCF7 breast cancer cells. Interestingly, cell death was increased dramatically by increasing irradiation dose from 8-32 J/cm(2) and then decreased by enhancing laser dose. The maximum amount of cell death is 50% which was observed in the presence of ZnO/rGO 20% by irradiation dose of 32 J/cm(2). Furthermore, in comparison with 810 nm, 630 nm lasers were more effective in LLLT of MCF7 cells. The results show the potential of using rGO-based NCs in LLLT, which may be combined with other therapeutic approaches to assist our fight against cancer.
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