期刊
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
卷 33, 期 -, 页码 456-461出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.algal.2018.06.013
关键词
Algal genome; Clustered cell-cycle; Culture synchronization; Flow cytometry; Multiple-fission cell-cycle; Mutational hazard hypothesis
资金
- European Union's Seventh Framework Programme (FP7), project GIAVAP [266401]
Studies revealed and keep revealing a staggering diversity of life-cycle patterns and genome-sizes in microalgae. Even within a single order like the Volvocales with Volvox carteri, Chlamydomonas reinhardtii, and Dunaliella salina, differences are significant. Additional knowledge gaps in microalgal physiology and genetics hinder our understanding of their evolution and valorization. Herein, we present for the first time, the multiple fission cell-cycle with corresponding DNA quantities of the economically important Volvocales Haematococcus pluvialis. Further, we provide two straightforward protocols for efficient culture synchronization, based on filtration or skimming of zoospores. The cell-cycle and nuclear DNA content where monitored by flow cytometry and fluorescence microscopy after staining with dsDNA dye PICO-or SYBR-green (R). The average nuclear DNA content was 3.6-4.1 and 22.6-25.2 pg in zoospores/coenobial daughter-cells and aplanospores, respectively. We found a single DNA replication step for all coenobial daughter cells prior mitosis, followed by one consecutive cytokinesis. Thus, our results suggest a new Haematococcus-type C-n cell-cycle with unusual polyploid and polynuclear phases. This cell-cycle regime might allow for the large genome-size of Haematococcus and is consistent with the mutational-hazard hypothesis for Volvocales microalgae.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据