期刊
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
卷 33, 期 -, 页码 16-27出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.algal.2018.04.028
关键词
Auxenochlorella protothecoides; Lipid accumulation; Temperature stress; Transcriptomics; iTRAQ-based proteomics; Fatty acid metabolism
资金
- National Natural Science Foundation of China [31570117]
- State Key Laboratory of Agrobiotechnology [2017SKLAB7-14]
Temperature is one of the critical environmental factors that influence microalgal growth, lipid content and fatty acid (FA) composition. However, the molecular mechanism underlying regulations of FA metabolism under low and high temperature stress in oleaginous microalgae remains unclear. In this study, integrated analyses of transcriptome, proteome and fatty acid profilings were performed for the first time in Auxenochlorella protothecoides UTEX 2341. Under low and high temperature (LT and HT) stress, a total of 5565 and 4757 genes, and 1311 and 728 proteins were differentially expressed respectively. 65 actively expressed genes and 61 proteins involved in FA metabolism were identified. A strong positive correlation between the genes' transcript and protein levels existed in FA metabolism (r = 0.80, p-value < 0.01**, LT vs NT; r = 0.61, p-value < 0.01**, HT vs NT). Two models were proposed to reveal differential reprogramming of FA metabolism induced by low and high temperatures. Low temperature promoted chloroplast FA biosynthesis by enhancing the expression of the plastidial acetyl-CoA carboxylase (ACCase) and type-II fatty acid synthase. High temperature activated FA biosynthesis, including polyunsaturated and very-long-chain FAs in the cytosol and endoplasmic reticulum (ER) by increasing the expression of the cytosolic ACCase, type-I polyketide synthase and components of the ER-located elongase complex. The enhanced expression of the plastid-located pyruvate dehydrogenase complex (PDHC) and the suppressed FA beta-oxidation also highly contributed to lipid accumulation. The biosynthesis of omega-3 fatty acid was closely related to microalgae's temperature adaptability. These results indicated that the reprogramming of FA metabolism was implicated in microalgae response to temperature stress. The above findings not only had important implications for the screening and genetic engineering of algae and plants to improve their lipid productions, but also provided novel insight into the adaptive mechanism to temperature stress.
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