期刊
POLYMERS
卷 10, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/polym10030292
关键词
arctic Chlorella sp.; polysaccharide; antioxidant activity; preliminary structural characterization
资金
- National Natural Science Foundation of China [31500318]
- Natural Science Foundation of Jiangsu Province [BK20140713]
The arctic strain of Chlorella sp. (Chlorella-Arc) exists in the coldest and driest arctic ecosystems, and it is a new resource of active polysaccharides. The extraction of crude polysaccharide from Chlorella-Arc was optimized using the response surface methodology. A crude polysaccharide yield of approximately 9.62 +/- 0.11% dry weight was obtained under these optimized conditions. Three fractions (P-I, P-II, and P-III) were present after purification by 2-diethylaminoethanol Sepharose Fast Flow and Sephadex G-100 chromatography. The P-IIa fraction demonstrated significant antioxidant activities. Moreover, P-IIa was an alpha-and beta-type heteropolysaccharide with a pyran group and contained variable amounts of rhamnose, arabinose, glucose, and galactose based on fourier-transform infrared spectroscopy, high-performance liquid chromatography, and H-1 and C-13 nuclear magnetic resonance imaging. Production of high amounts of polysaccharide may allow further exploration of the microalgae Chlorella-Arc as a natural antioxidant.
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