期刊
OPEN BIOLOGY
卷 8, 期 4, 页码 -出版社
ROYAL SOC
DOI: 10.1098/rsob.170271
关键词
cationic nanoparticles; lysosomal membrane permeabilization; autophagy; reactive oxygen species; nanotoxicity
资金
- ESF Research Networking Programme EpitopeMap
- University of Strasbourg Institute for Advanced Study (USIAS)
- TRANSAUTOPHAGY COST Action [CA15138]
Nanoparticles (NPs) typically accumulate in lysosomes. However, their impact on lysosomal function, as well as autophagy, a lysosomal degradative pathway, is still not well known. We have previously reported in the 1321N1 cell line that amine-modified polystyrene (NH2-PS) NPs induce apoptosis through damage initiated in the lysosomes leading ultimately to release of lysosomal content in the cytosol, followed by apoptosis. Here, by using a combination of biochemical and cell biological approaches, we have characterized in a mouse embryonic fibroblast cell line that the lysosomal alterations induced by NH2-PS NPs is progressive, initiating from mild lysosomal membrane permeabilization (LMP), to expansion of lysosomal volume and intensive LMP before the summit of cell death. Though the cells initially seem to induce autophagy as a surviving mechanism, the damage of NH2-PS NPs to lysosomes probably results in lysosomal dysfunctions, leading to blockage of autophagic flux at the level of lysosomes and the eventual cell death.
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