期刊
JOURNAL OF SAUDI CHEMICAL SOCIETY
卷 23, 期 2, 页码 222-238出版社
ELSEVIER
DOI: 10.1016/j.jscs.2018.06.011
关键词
CuONPs; ROS; Apoptosis; Cytokines level; Pro & anti apoptotic proteins
资金
- Department of Science and Technology (DST) through the INSPIRE Fellowship scheme [DST/INSPIRE Fellowship/2015/IF150762]
Green nanotechnology elucidates highly prioritized anticancer activity. We synthesized Copper oxide nanoparticles (CuONPs) using leaves of Azadirachta indica (A. indica) plants and studied the molecular mechanism of cancer cell apoptosis. After their synthesis, with the help of expository tools like Fourier transform infrared spectroscopy (FT-IR), Transmission electron microscopy (TEM), Dynamic light scattering (DLS) and surface zeta potential we confirmed the successful synthesis of CuONPs. Here, crystalline structure of green synthesized CuONPs of 36 +/- 8 nm size and spherical shape was able to kill MCF-7 and Hela cells, estimated by MTT assay. Successful internalization of Cu+2 ions inside the cell was estimated by the atomic absorption study. Cellular uptake of Cu+2 ions inflicted significant Reactive Oxygen Species (ROS) generation inside the cancer cells, thereby leading to DNA fragmentation as observed by DAPI staining. In in vivo model, CuONPs reduced the breast tumor volume in Balb/C mice and increased the mean survival time through the alteration of pro-inflammatory cytokines level. In case of both in vivo and in vitro models, CuONPs altered the pro-inflammatory cytokine level and pro-apoptotic protein expressions. In future, green synthesized CuONPs might be beneficial for its application as an anticancer drug in in vivo (mice model) and in vitro, though further study is needed on its toxicity. (C) 2018 King Saud University. Production and hosting by Elsevier B.V.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据