期刊
ACS APPLIED MATERIALS & INTERFACES
卷 7, 期 8, 页码 4728-4736出版社
AMER CHEMICAL SOC
DOI: 10.1021/am508421n
关键词
supramolecular assembly; DTAB; pyrene; ratiometric sensor; pepsin
资金
- National Natural Science Foundation of China [21173142]
- Ministry of Education of China [NCET-12-0895]
- Shaanxi Provincial Department of Science and Technology [2011KJXX48]
- Program of Introducing Talents of Discipline to Universities [B14041]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT_14R33]
- Fundamental Research Funds for the Central Universities [GK201301006]
Novel strategies of developing fluorescent sensors for proteins are highly demanded. In this work, we particularly synthesized a cholesterol-derivatized pyrene probe. Its fluorescence emission is effectively tuned by the aggregation state of a cationic surfactant dodecyltrimethylammonium bromide (DTAB). The used probe/DTAB assemblies exhibit highly sensitive ratiometric responses to pepsin and ovalbumin egg (o-egg) with detection limits of 4.8 and 18.9 nM, respectively. The fluorescence changes indicate the protein-surfactant interaction leads to further aggregation of DTAB assemblies. The results from Tyndall effect and dynamic light scattering verify this assumption. The responses to pepsin and o-egg are due to their strong electrostatic or hydrophobic interaction with DTAB assemblies at pH 7.4. The present noncovalent supramolecular sensor represents a novel and simple strategy for sensing proteins, which is based on the encapsulated fluorophore probing the aggregation variation of the surfactant assemblies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据