Determination of Antioxidants by Sensitized Chemiluminescence Using 2,2′-azo-bis(2-amidinopropane)

An analytical procedure for determining antioxidants was proposed based on chemiluminescence. The analytical system consisted of a free-radicals generator, 2,2'-azo-bis(2-amidinopropane), luminol was used as a chemiluminescence sensitizer in phosphate buffer solution (pH 7.4). The analytical conditions were optimized. The analytical procedure detection limits as low as (mu M) the following: for trolox, the limit is 0.05; for ascorbate, it is 0.20; for quercetin, it is 0.03; and, for dihydroquercetin, it is 0.03. A test determination of antioxidants in food was performed.