期刊
CELL REPORTS
卷 23, 期 7, 页码 2199-2210出版社
CELL PRESS
DOI: 10.1016/j.celrep.2018.04.061
关键词
-
类别
资金
- ERC [339953]
- Lundbeck Foundation
- Novo Nordisk Foundation
Mammalian genomes are promiscuously transcribed, yielding protein-coding and non-coding products. Many transcripts are short lived due to their nuclear degradation by the ribonucleolytic RNA exosome. Here, we show that abolished nuclear exosome function causes the formation of distinct nuclear foci, containing polyadenylated (pA(+)) RNA secluded from nucleocytoplasmic export. We asked whether exosome co-factors could serve such nuclear retention. Co-localization studies revealed the enrichment of pA(+) RNA foci with ''pA-tail exosome targeting (PAXT) connection'' components MTR4, ZFC3H1, and PABPN1 but no overlap with known nuclear structures such as Cajal bodies, speckles, paraspeckles, or nucleoli. Interestingly, ZFC3H1 is required for foci formation, and in its absence, selected pA(+) RNAs, including coding and non-coding transcripts, are exported to the cytoplasm in a process dependent on the mRNA export factor AlyREF. Our results establish ZFC3H1 as a central nuclear pA(+) RNA retention factor, counteracting nuclear export activity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据