期刊
CELL REPORTS
卷 22, 期 7, 页码 1923-1934出版社
CELL PRESS
DOI: 10.1016/j.celrep.2018.01.056
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资金
- NIH [R01GM115710, R01GM122926]
- CMB TG [T32GM007223, F31AG058405, F31DE026946]
- Yale Cancer Center
- NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH [F31DE026946] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [T32GM007223, R01GM115710, R01GM122926] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [F31AG058405] Funding Source: NIH RePORTER
Ribosome biogenesis is a highly regulated, essential cellular process. Although studies in yeast have established some of the biological principles of ribosome biogenesis, many of the intricacies of its regulation in higher eukaryotes remain unknown. To understand how ribosome biogenesis is globally integrated in human cells, we conducted a genome-wide siRNA screen for regulators of nucleolar number. We found 139 proteins whose depletion changed the number of nucleoli per nucleus from 2-3 to only 1 in human MCF10A cells. Follow-up analyses on 20 hits found many (90%) to be essential for the nucleolar functions of rDNA transcription (7), pre-ribosomal RNA (pre-rRNA) processing (16), and/or global protein synthesis (14). This genome-wide analysis exploits the relationship between nucleolar number and function to discover diverse cellular pathways that regulate the making of ribosomes and paves the way for further exploration of the links between ribosome biogenesis and human disease.
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