期刊
CELL REPORTS
卷 22, 期 11, 页码 3087-3098出版社
CELL PRESS
DOI: 10.1016/j.celrep.2018.02.063
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资金
- ERC (NEURO-PATTERNS)
- NIH [1U01NS090576-01]
- FP7 (DESIRE)
- Flag-Era JTC Human Brain Project (SLOW-DYN)
Sensory information is encoded within the brain in distributed spatiotemporal patterns of neuronal activity. Understanding how these patterns influence behavior requires a method to measure and to bidirectionally perturb with high spatial resolution the activity of the multiple neuronal cell types engaged in sensory processing. Here, we combined two-photon holography to stimulate neurons expressing blue light-sensitive opsins (ChR2 and GtACR2) with two-photon imaging of the red-shifted indicator jRCaMP1a in the mouse neocortex in vivo. We demonstrate efficient control of neural excitability across cell types and layers with holo-graphic stimulation and improved spatial resolution by opsin somatic targeting. Moreover, we performed simultaneous two-photon imaging of jRCaMP1a and bidirectional two-photon manipulation of cellular activity with negligible effect of the imaging beam on opsin excitation. This all-optical approach represents a powerful tool to causally dissect how activity patterns in specified ensembles of neurons determine brain function and animal behavior.
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