期刊
CELL REPORTS
卷 23, 期 4, 页码 983-992出版社
CELL PRESS
DOI: 10.1016/j.celrep.2018.03.113
关键词
-
类别
资金
- Swiss National Science Foundation [31003A_149463]
- Republic and Canton of Geneva
- University of Geneva
- Swiss National Science Foundation (SNF) [31003A_149463] Funding Source: Swiss National Science Foundation (SNF)
The Saccharomyces cerevisiae telomere-binding protein Rift plays an evolutionarily conserved role in control of DNA replication timing by promoting PP1-dependent dephosphorylation of replication initiation factors. However, ScRif1 binding outside of telomeres has never been detected, and it has thus been unclear whether Rif1 acts directly on the replication origins that it controls. Here, we show that, in unperturbed yeast cells, Rif1 primarily regulates late-replicating origins within 100 kb of a telomere. Using the chromatin endogenous cleavage ChEC-seq technique, we robustly detect Rif1 at late-replicating origins that we show are targets of its inhibitory action. Interestingly, abrogation of Rif1 telomere association by mutation of its Rap1-binding module increases Rif1 binding and origin inhibition elsewhere in the genome. Our results indicate that Rif1 inhibits replication initiation by interacting directly with origins and suggest that Rap1-dependent sequestration of Rif1 increases its effective concentration near telomeres, while limiting its action at chromosome-internal sites.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据