期刊
CELL REPORTS
卷 22, 期 10, 页码 2767-2783出版社
CELL PRESS
DOI: 10.1016/j.celrep.2018.02.042
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资金
- People Programme (Marie Curie Actions) of the European Union's Seventh Framework Programme (FP7) under REA grant [PII-GA-2012-330061]
- Swiss National Science Foundation [133710]
- European Research Council (OPTOMOT)
- New York Stem Cell Foundation
Optogenetic tools and imaging methods for recording and manipulating brain activity have boosted the field of neuroscience in unprecedented ways. However, behavioral paradigms for mice lag behind those of primates, limiting the full potential of such tools. Here, we present an innovative behavioral framework in which head-fixed mice directionally reach for water droplets, similar to the primate center-out'' reaching task. Mice rapidly engaged in the task, performed hundreds of trials, and reached in multiple directions when droplets were presented at different locations. Surprisingly, mice used chemosensation to determine the presence of water droplets. Optogenetic inactivation of the motor cortex halted the initiation and rapidly diverted the trajectory of ongoing movements. Layer 2/3 two-photon imaging revealed robust direction selectivity in most reach-related neurons. Finally, mice performed directional reaching instructed by vibrato-tactile stimuli, demonstrating the potential of this framework for studying, in addition to motor control, sensory processing, and decision making.
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