期刊
ADVANCED HEALTHCARE MATERIALS
卷 7, 期 12, 页码 -出版社
WILEY
DOI: 10.1002/adhm.201800122
关键词
3D cell culture model; 3D endothelium model; microfluidics; organ-on-a-chip
资金
- National Institute of Health [1R21CA182375-01A1]
- Baker Fellows Program
- National Science Foundation [1509921]
- Jungsong Fellowship
- Div Of Electrical, Commun & Cyber Sys [1509921] Funding Source: National Science Foundation
- NATIONAL CANCER INSTITUTE [R21CA182375] Funding Source: NIH RePORTER
Engineering physiologically relevant in vitro models of human organs remains a fundamental challenge. Despite significant strides made within the field, many promising organ-on-a-chip models fall short in recapitulating cellular interactions with neighboring cell types, surrounding extracellular matrix (ECM), and exposure to soluble cues due, in part, to the formation of artificial structures that obstruct >50% of the surface area of the ECM. Here, a 3D cell culture platform based upon hydrophobic patterning of hydrogels that is capable of precisely generating a 3D ECM within a microfluidic channel with an interaction area >95% is reported. In this study, for demonstrative purposes, type I collagen (COL1), Matrigel (MAT), COL1/MAT mixture, hyaluronic acid, and cell-laden MAT are formed in the device. Three potential applications are demonstrated, including creating a 3D endothelium model, studying the interstitial migration of cancer cells, and analyzing stem cell differentiation in a 3D environment. The hydrophobic patterned-based 3D cell culture device provides the ease-of-fabrication and flexibility necessary for broad potential applications in organ-on-a-chip platforms.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据