期刊
ADVANCED HEALTHCARE MATERIALS
卷 7, 期 9, 页码 -出版社
WILEY
DOI: 10.1002/adhm.201701249
关键词
biofabrication; bioprinting; microwells; poly(ethylene glycol); tissue engineering
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC) [RGPIN-2014-04010, RGPIN-341487-12]
- Canadian Foundation for Innovation John R. Evans Leaders Opportunity Fund
A direct laser bioprinting (DLBP) system is introduced in this work. The DLBP system applies visible-laser-induced photo-crosslinking at a wavelength of 405 nm using the photoinitiator VA-086. It is shown that such a system can fabricate vertical structures with fine features (less than 50 mu m) and high cell viability (greater than 95%). Experimental characterizations and theoretical simulations are presented, and good agreement is seen between the experiments and theory. The DLBP system is applied to the fabrication of (1) cell-laden hydrogel microgrids, (2) hydrogel microwells, as well as a test of (3) cell encapsulation, and (4) cell seeding. The DLBP system is found to be a promising tool for bioprinting.
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