期刊
ADVANCED HEALTHCARE MATERIALS
卷 7, 期 16, 页码 -出版社
WILEY
DOI: 10.1002/adhm.201701459
关键词
biosensors; cytotoxicity; dopamine; PC12; living cells; nitrogen-doped mesoporous carbon nanosheets
3D-ridge nanosheets of N-doped mesoporous carbon (NMCS)-based electrodes are fabricated as ultrasensitive biosensors for in vitro monitoring of dopamine (DA) released from living cells. The large-scale ranges of dense-layered sheets are arranged linearly with a thickness of <10 nm, soft tangled edges, stocked layer arrangements, and tunable mesoporous frameworks with 3D orientations. The intrinsic features of the active interfacial surface of the electrode based on NMCS along with polarized surfaces, dense surface-charged matrices, fast electron transfer, and easy molecular diffusion, are present in the highly active electrode for biosensing applications. The designed electrode based on the NMCS shows high sensitivity and selectivity for DA sensing even in the presence of physiological interference molecules, such as ascorbic acid and/or uric acid, at a low applied potential of 0.25 V versus Ag/AgCl. The large-scale NMCS-based electrode shows low detection limits as low as 10 nmol L-1, wide linear range up to 0.5 mmol L-1, long-term stability for more than 15 d (relative standard deviation (RSD)= 5.8%), and a low cytotoxicity with high biocompatibility. The findings demonstrated that the NMCS-based electrode is a reliable modified electrode for ultratrace sensitivity of DA, which is secreted normally from dopaminergic cells (PC12) or under a stimulating agent (K+).
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据