期刊
ACS SYNTHETIC BIOLOGY
卷 7, 期 3, 页码 945-951出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.8b00045
关键词
giant unilamellar vesicles; thylakoid; electron transfer; light reaction; artificial cells
资金
- National Natural Science Foundation of China [21773050, 21528501]
- Harbin Distinguished Young Scholars Fund [2017RAYXJ024]
The fabrication of artificial cells containing nature components is challenging. Herein we construct a thylakoid containing artificial cell (TA-cell) by forming multicompartmental structure inside giant unilamellar vesicles (GUVs) using osmotic stress. The thylakoids are selectively loaded inside each compartment in GUVs to mimic chloroplast. The TA-cells are able to carry out photosynthesis upon light on. The TA-cells keep their 50% functionality of electron transfer for 12 days which is twice of those of free thylakoids. Using TA-cells the inhibition of 3-(34-dichlorophenyl)-11-dimethylurea (DCMU) and heavy metal ions (Hg2+ Cu2+ Cd2+ Pb2+ and Zn2+) on the electron transfer process in TA-cells is systematically investigated. Their half maximal inhibitory concentration (IC50) values are 36.23 +/- 1.87 0.02 +/- 0.01 0.42 +/- 0.08 0.82 +/- 0.12 1.97 +/- 0.21 and 4.08 +/- 0.18 mu M respectively. Hg2+ is the most toxic ion for the photosynthesis process among these five heavy metal ions. This biomimetic system can be expanded to study other processes during the photosynthesis. The TA-cells pave a way to fabricate more complicated nature component containing artificial cells.
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