期刊
ACS SYNTHETIC BIOLOGY
卷 7, 期 2, 页码 299-310出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.7b00308
关键词
serine integrase; recombination directionality factor (RDF); DNA assembly; genome engineering logic devices; genetic data storage
资金
- UK Engineering and Physical Sciences Research Council [EP/H019154/1, EP/K034359/1]
- BBSRC [BB/M018229/1, BB/M018237/1, BB/K003356/1, BB/M018040/1] Funding Source: UKRI
- EPSRC [EP/K034359/1, EP/H019154/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/M018229/1, BB/M018040/1, BB/K003356/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/K034359/1, EP/H019154/1] Funding Source: researchfish
Serine integrases catalyze precise rearrangement of DNA through site-specific recombination of small sequences of DNA called attachment (att) sites. Unlike other site-specific recombinases, the recombination reaction driven by serine integrases is highly directional and can only be reversed in the presence of an accessory protein called a recombination directionality factor (RDF). The ability to control reaction directionality has led to the development of serine integrases as tools for controlled rearrangement and modification of DNA in synthetic biology, gene therapy, and biotechnology. This review discusses recent advances in serine integrase technologies focusing on their applications in genome engineering, DNA assembly, and logic and data storage devices.
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