期刊
ACS SYNTHETIC BIOLOGY
卷 7, 期 3, 页码 767-773出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.7b00278
关键词
synthetic viruses; DNA origami; green fluorescent protein; transfection; microscopy
资金
- UK's Department for Business, Energy and Industrial Strategy
- UK's Engineering and Physical Sciences Research Council (EPSRC) [EP/M506448/1]
- MRC [MR/R024871/1] Funding Source: UKRI
A synthetic topology for everted viruses is reported. The topology is a single-stranded virion DNA assembled into a hollow cube with exterior decorated with HIV-Tat transduction domains. The cube incorporates a pH responsive lid allowing for the controlled encapsulation of functional proteins and their transfer and release into live cells. Unlike viruses, which are protein shells with a [3,S]-fold rotational symmetry that encase nucleic acids, these cubes are [3, 4]-fold DNA boxes encapsulating proteins. Like viruses, such everted DNA-built viruses are monodisperse nanoscale assemblies that infect human cells with a specialist cargo. The design offers a bespoke bottom-up platform for engineering nonpolyhedral, nonprotein synthetic viruses.
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