期刊
THERANOSTICS
卷 8, 期 5, 页码 1449-1457出版社
IVYSPRING INT PUBL
DOI: 10.7150/thno.22540
关键词
gold nanoparticles; D-amino acid; bacteria; spontaneous bacterial peritonitis; colorimetric biosensor
资金
- Ministry of Science and Technology of China [2013YQ190467]
- Chinese Academy of Sciences [XDA09030305]
- National Science Foundation of China [81361140345, 51373043, 21535001]
Rationale: Rapid and facile detection of pathogenic bacteria is challenging due to the requirement of large-scale instruments and equipment in conventional methods. We utilize D-amino acid as molecules to selectively target bacteria because bacteria can incorporate DADA in its cell wall while mammalian cells or fungi cannot. Methods: We show a broad-spectrum bacterial detection system based on D-amino acid-capped gold nanoparticles (AuNPs). AuNPs serve as the signal output that we can monitor without relying on any complex instruments. Results: In the presence of bacteria, the AuNPs aggregate and the color of AuNPs changes from red to blue. This convenient color change can distinguish between Staphylococcus aureus (S. aureus) and methicillin-resistant Staphylococcus aureus (MRSA). This system can be applied for detection of ascites samples from patients. Conclusion: These D-amino acid-modified AuNPs serve as a promising platform for rapid visual identification of pathogens in the clinic.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据