期刊
STEM CELL RESEARCH & THERAPY
卷 9, 期 -, 页码 -出版社
BIOMED CENTRAL LTD
DOI: 10.1186/s13287-018-0819-z
关键词
Premature ovarian failure; CP-MSCs; Transplantation; Cyclophosphamide; Ovarian function
资金
- National Natural Science Foundation of China [81473172, 81503025, 81720108030]
- Program for Scientific Research Innovation Team in Colleges and Universities of Jiangsu Province
- Jiangsu Province Postdoctoral Foundation [1701068C]
- Postdoctoral Foundation of China [2017M621658, 2017M621659]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
Background: Previous studies have reported that transplantation of mesenchymal stem cells (MSCs) from many human tissues could ameliorate ovarian dysfunction. However, no study has revealed the therapeutic efficiency of MSCs derived from the chorionic plate (CP-MSCs) for premature ovarian failure (POE).& para;& para;Methods: We investigated the restorative effects of CP-MSCs on cyclophosphamide (CTX)-induced POF. The POF mouse models were established via intraperitoneal injection of 50 mg/kg CTX into female mice for 15 consecutive days. After that, CP-MSCs were intravenously transplanted into the mice once a week for 4 weeks. The serum estradiol (E2) and follicle-stimulating hormone (FSH) levels in the mouse models were detected using enzyme-linked immunosorbent assay (ELISA) before and after treatment. Ovarian function was evaluated through counting the follicles, estrous cycles, and oocytes.& para;& para;Results: CP-MSC transplantation restored the serum hormone level and ovarian function of the mice in the mouse model of POF induced by CTX. The levels of serum E2 and FSH in the POF model group was 232.33 +/- 17.16 pg/mL and 4.48 +/- 0.29 mIU/mL, respectively, after 6 weeks of treatment, which were similar to the values in the wild-type (WT) group. The superovulation demonstrated that ovarian function was significantly improved compared with nontreated POF model mice. The CP-MSC transplantation could restore CTX-induced ovarian dysfunction.& para;& para;Conclusions: Our results offer a potential application for human CP-MSCs in POF treatment.
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