期刊
SCIENTIFIC REPORTS
卷 8, 期 -, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41598-018-27682-w
关键词
-
资金
- Mineco [SAF2014-55399-R, SAF2014-55523-R, SAF2016-77222, SAF2017-84494-C2-1R]
- Instituto de Salud Carlos III [PI15/01100]
- European Union (Fondo Europeo de Desarrollo Regional FEDER)
- Universidad Complutense, Fondo de Garantia Juvenil (Comunidad de Madrid)
- Ciberes grant
- Fundacion Contra la Hipertension Pulmonar
- FPU grant from Ministerio de Educacion
- People Programme (Marie Curie Actions) of the FP7 under REA [600396]
- MEIC-AEI
- Pro CNIC Foundation
- Severo Ochoa Center of Excellence (MEIC) [SEV-2015-0505]
We have analysed whether pulmonary arterial hypertension (PAH) alters the rat faecal microbiota. Wistar rats were injected with the VEGF receptor antagonist SU5416 (20 mg/kg s.c.) and followed for 2 weeks kept in hypoxia (10% O-2, PAH) or injected with vehicle and kept in normoxia (controls). Faecal samples were obtained and microbiome composition was determined by 165 rRNA gene sequencing and bioinformatic analysis. No effect of PAH on the global microbiome was found (alpha- or beta-diversity). However, PAH-exposed rats showed gut dysbiosis as indicated by a taxonomy-based analysis. Specifically, PAH rats had a three-fold increase in Firmicutes-to-Bacteroidetes ratio. Within the Firmicutes phylum, there were no large changes in the relative abundance of the bacterial families in PAH. Among Bacteroidetes, all families were less abundant in PAH. A clear separation was observed between the control and PAH clusters based on short chain fatty acid producing bacterial genera. Moreover, acetate was reduced in the serum of PAH rats. In conclusion, faecal microbiota composition is altered as a result of PAH. This misbalanced bacterial ecosystem might in turn play a pathophysiological role in PAH by altering the immunologic, hormonal and metabolic homeostasis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据