γδTCR regulates production of interleukin-27 by neutrophils and attenuates inflammatory arthritis

gamma delta T cells have been implicated in inflammatory diseases as an important link between the innate and adaptive immune responses, however, their role in inflammatory arthritis remain unclear. To define the contribution of gamma delta T cells in the pathogenesis of inflammatory arthritis, we performed gene transfer of IL-23 in B10. RIII mice to establish joint inflammation in the presence or absence of gamma delta T cells. We demonstrated that gamma delta T cell blockade has a protective effect on arthritis incidence and severity by preventing neutrophil accumulation in the blood, spleen and bone marrow as well as by reducing neutrophil infiltration into the joints. Furthermore, our data demonstrate that absence of gamma delta T cells was associated with an increase of IL-27 levels produced by neutrophils and dendritic cells, and systemic IL-27 expression also prevents IL-23-induced inflammatory arthritis and limits neutrophil expansion. Collectively our findings reveal an immunomodulatory effect of gamma delta T cells on neutrophils associated with IL-27 synthesis and secretion and indicate a novel link between IL-27 and the modulation of gamma delta T cells and neutrophils that can be targeted in the treatment of inflammatory arthritis.