期刊
SCIENTIFIC REPORTS
卷 8, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-018-24964-1
关键词
-
资金
- Spanish Ministry of Economy and Competitiveness [AGL2013-45339-R, SAF2014-57856]
- Catalonian government [2017SGR499]
- US National Institutes of Health [R01 GM038784]
- UB (APIF)
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM038784] Funding Source: NIH RePORTER
Metal limitation is a common situation during infection and can have profound effects on the pathogen's success. In this report, we examine the role of zinc limitation in the expression of a virulence factor in uropathogenic Escherichia coli. The pyelonephritis isolate J96 carries two hlyCABD operons that encode the RTX toxin alpha-hemolysin. While the coding regions of both operons are largely conserved, the upstream sequences, including the promoters, are unrelated. We show here that the two hlyCABD operons are differently regulated. The hly(II) operon is efficiently silenced in the presence of zinc and highly expressed when zinc is limited. In contrast, the hly(I) operon does not respond to zinc limitation. Genetic studies reveal that zinc-responsive regulation of the hly(II) operon is controlled by the Zur metalloregulatory protein. A Zur binding site was identified in the promoter sequence of the hlyII operon, and we observe direct binding of Zur to this promoter region. Moreover, we find that Zur regulation of the hlyII operon modulates the ability of E. coli J96 to induce a cytotoxic response in host cell lines in culture. Our report constitutes the first description of the involvement of the zinc-sensing protein Zur in directly modulating the expression of a virulence factor in bacteria.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据