期刊
SCIENTIFIC REPORTS
卷 8, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-017-18725-9
关键词
-
资金
- FEDER funds through the COMPETE Programme
- FCT - Portuguese Foundation for Science and Technology [POCI-01-0145-FEDER-007688, UID/CTM/50025/2013]
- project DISERTOX [PTDC/CTM-NAN/2912/2014]
- Unidade de Ciencias Biomoleculares Aplicadas - UCIBIO - FCT/MCTES [UID/Multi/04378/2013]
- ERDF [POCI-01-0145-FEDER-007728, PD/BD/52211/2013]
- Fundação para a Ciência e a Tecnologia [PD/BD/52211/2013] Funding Source: FCT
Many diseases have their treatment options narrowed and end up being fatal if detected during later stages. As a consequence, point-of-care devices have an increasing importance for routine screening applications in the health sector due to their portability, fast analyses and decreased cost. For that purpose, a multifunctional chip was developed and tested using gold nanoprobes to perform RNA optical detection inside a microfluidic chip without the need of molecular amplification steps. As a proof-of-concept, this device was used for the rapid detection of chronic myeloid leukemia, a hemato-oncological disease that would benefit from early stage diagnostics and screening tests. The chip passively mixed target RNA from samples, gold nanoprobes and saline solution to infer a result from their final colorimetric properties. An optical fiber network was used to evaluate its transmitted spectra inside the chip. Trials provided accurate output results within 3 min, yielding signal-to-noise ratios up to 9 dB. When compared to actual state-of-art screening techniques of chronic myeloid leukemia, these results were, at microscale, at least 10 times faster than the reported detection methods for chronic myeloid leukemia. Concerning point-of-care applications, this work paves the way for other new and more complex versions of optical based genosensors.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据