Effects of combined pretreatment of dilute acid pre-extraction and chemical-assisted mechanical refining on enzymatic hydrolysis of lignocellulosic biomass

An efficient enzymatic hydrolysis of lignocellulosic biomass into fermentable sugars depends greatly on the pretreatment of raw materials. In this study, a combination of dilute acid pre-extraction and chemical-assisted mechanical refining was used to pretreat wood lignocellulosic biomass for subsequent enzymatic hydrolysis. This work analyzed the surface lignin concentration, specific surface area, crystallinity, fines content, fiber length, and kink index of the resultant pulp substrates and their effects on the enzymatic hydrolysis. The results showed that the combined pretreatment significantly enhanced the enzymatic hydrolysis efficiency, and the maximum glucose conversion yield and glucose concentration were 93.32% and 21.41 g L-1, respectively. It is found that the surface lignin concentration, specific surface area, and fines content significantly affected the enzymatic hydrolysis.