期刊
RSC ADVANCES
卷 8, 期 18, 页码 9603-9610出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8ra00849c
关键词
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资金
- Engineering and Physical Sciences Council (UK) [EP/G501408/1]
- Unilever CASE Award (UK) [EP/G501408/1]
We report upon a novel coplanar dielectrophoresis (DEP) based cell patterning system for generating transferrable hepatic cell constructs, resembling a liver-lobule, in culture. The use of paper reinforced gel substrates provided sufficient strength to enable these constructs to be transfered into 96-well plates for long term functional studies, including in the future, drug development studies. Experimental results showed that hepatic cells formed DEP field-induced structures corresponding to an array of lobule-mimetic patterns. Hepatic viability was observed over a period of 3 days by the use of a fluorescent cell staining technique, whilst the liver specific functionality of albumin secretion showed a significant enhancement due to the layer patterning of cell lines (HepG2/C3A), compared to 2D patterned cells and un-patterned control. This build and transfer concept could, in future, also be adapted for the layer-by-layer construction of organs-on-chip in microtitre formats.
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