期刊
NUTRIENTS
卷 10, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/nu10070857
关键词
strawberry; food allergy; Fra a 1; pathogenesis-related proteins; indirect competitive ELISA
资金
- Bundesanstalt fur Landwirtschaft und Ernahrung (BLE) grant [14OE008]
Birch pollen allergic patients show cross-reactivity to vegetables and fruits, including strawberries (Fragaria x ananassa). The objective of this study was to quantify the level of the Fra a 1 protein, a Bet v 1-homologous protein in strawberry fruits by a newly developed ELISA, and determine the effect of genotype, cultivation and food processing on the allergen amount. An indirect competitive ELISA using a specific polyclonal anti-Fra a 1.02 antibody was established and revealed high variability in Fra a 1 levels within 20 different genotypes ranging from 0.67 to 3.97 mu g/g fresh weight. Mature fruits of red-, white- and yellow-fruited strawberry cultivars showed similar Fra a 1 concentrations. Compared to fresh strawberries, oven and solar-dried fruits contained slightly lower levels due to thermal treatment during processing. SDS-PAGE and Western blot analysis demonstrated degradation of recombinant Fra a 1.02 after prolonged (>10 min) thermal treatment at 99 degrees C. In conclusion, the genotype strongly determined the Fra a 1 quantity in strawberries and the color of the mature fruits does not relate to the amount of the PR10-protein. Cultivation conditions (organic and conventional farming) do not affect the Fra a 1 level, and seasonal effects were minor.
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