期刊
FOOD & FUNCTION
卷 9, 期 1, 页码 407-416出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7fo01568b
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资金
- Enterprise Ireland [TC2013-0001]
- ERASMUS Program
Tropical banded crickets (Gryllodes sigillatus) were studied for their ability to yield hydrolysates with dipeptidyl peptidase IV (DPP-IV) inhibitory properties. A cricket protein isolate (CPI) was prepared following extraction of the water soluble proteins from G. sigillatus powder (CP). The extraction yield and purity were 20.90 +/- 0.35% and 57.0 +/- 2.23%, respectively. Endogenous proteinase activities were detected in the CP, which were linked to the significant protein breakdown seen in this sample. Fifteen CPI hydrolysates (H1-H15) were generated with Protamex(TM) using a design of experiments (DOE) approach combining three parameters, temperature (40, 50 and 60 degrees C), enzyme to substrate ratio (E : S, 0.50, 1.25 and 2.00% (w/w)) and hydrolysis time (60, 150 and 240 min). The DPP-IV half maximal inhibitory concentrations (IC50) of the CPI hydrolysates ranged from 0.40 +/- 0.03/0.40 +/- 0.02 (H2/H3) to 1.01 +/- 0.07 mg mL(-1) (H7). Following simulated gastrointestinal digestion (SGID), the DPP-IV IC50 of CPI decreased (> 3.57 vs. 0.78 +/- 0.04 mg mL(-1)) while that of H5 increased (0.47 +/- 0.03 vs. 0.71 +/- 0.06 mg mL(-1)). This study has demonstrated for the first time that G. sigillatus protein hydrolysates are able to inhibit DPP-IV. The study of these hydrolysates in vivo is needed to evaluate their potential role in glycaemic management.
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