期刊
CONSERVATION GENETICS RESOURCES
卷 12, 期 1, 页码 17-19出版社
SPRINGER
DOI: 10.1007/s12686-018-1054-8
关键词
Acipenser fulvescens; qPCR; eDNA; Quantification; Species at risk
To assist in efforts to monitor the distribution and abundance of lake sturgeon Acipenser fulvescens, a species of conservation concern in Canada and the United States, we developed two quantitative PCR (qPCR) environmental DNA assays targeting the cytochrome c oxidase 1 (COI) and cytochrome b (cyt b) genes. Neither assay amplified DNA from the closely-related shortnose sturgeon Acipenser brevirostrum, but species-specificity should be tested further. In field and laboratory trials, results from the cyt b assay always corresponded with species presence; the COI assay occasionally yielded false positives. The cyt b assay also showed promise in estimating relative abundance; C-t value (the number of PCR cycles at which DNA detection exceeds the background level) decreased with density in both the field and lab.
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